High fidelity restriction endonucleases
AssigneeNew England Biolabs, Inc.
InventorsZhenyu Zhu, Aine Quimby, Shengxi Guan, Dapeng Sun, Yishu Huang, Xuhui Lai, Siu-hong Chan, Xianghui Li, Shuang-Yong Xu, Chunhua Zhang
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Abstract
Methods and compositions are provided for engineering mutant enzymes with reduced star activity where the mutant enzymes have a fidelity index (FI) in a specified buffer that is greater than the FI of the non-mutated enzyme in the same buffer.
Claims2
A composition comprising a variant NsiI restriction endonuclease having reduced star activity, wherein the variant NsiI restriction endonuclease comprises an amino acid sequence that differs from the amino acid sequence of the parent NsiI restriction endonuclease by an amino acid substitution at a position corresponding to position 376 in SEQ ID NO:10.
Dependencies are inferred from the claim text.