Modified Bacillus cereus phospholipase C protein and method of processing vegetable oil

Abstract

A modified Bacillus cereus phospholipase C enzyme is provided, as well as a method of using the modified phospholipase C enzyme in a method of treating vegetable oil. In certain embodiments, this method may comprise combining a vegetable oil with an modified phospholipase C enzyme comprising an amino acid sequence that is at least 85% identical to the amino acid sequence of SEQ ID NO:1, wherein the amino acid residue at position 66 is a Trp (W) or Tyr (Y), and maintaining the combination under conditions suitable for the modified phospholipase C enzyme to catalyze the hydrolysis of phospholipids in the oil to produce diacylglycerol and a water soluble phosphate.

Dependencies are inferred from the claim text.