Modified carbonyl reducing enzyme and gene
AssigneeKaneka Corporation
InventorsMisato Matsui, Noriyuki Ito, Shigeru Kawano, Yoshihiko Yasohara
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Abstract
An object of the present invention is to modify a wild-type enzyme that is less reactive in the presence of an organic solvent to provide altered carbonyl reductases having better reactivity in the presence of the organic solvent than the wild-type enzyme, and/or to provide transformants producing such reductases. The present inventors have found altered carbonyl reductases having better reactivity in the presence of an organic solvent than the wild-type enzyme, from among a mutant enzyme library prepared by randomly mutating the wild-type enzyme gene, thereby arriving at completion of the present invention.
Claims7
An altered carbonyl reductase having the following properties (a) to (c):
- (a) an amino acid sequence having at least 85% sequence identity to the amino acid sequence of SEQ ID NO:1;
- (b) reduces 2-pentanone into 2-pentanol;
- (c) higher activity to reduce carbonyl compound in the presence of an organic solvent compared to the activity of the carbonyl reductase of SEQ ID NO:1 wherein the organic solvent is dimethylformamide, and wherein the altered carbonyl reductase comprises an amino acid substitution, relative to the amino acid sequence of SEQ ID NO:1, at one or more positions selected from the group-consisting of positions 2, 22, 25, 39, 42, 45, 51, 56, 71, 87, 90, 102, 109, 124, 135, 138, 155, 159, 175, 177, 183, 190, 195, 212, 220, 226, 228, 236, 238, 250, 254, 257, 259, 265, 267, 270, 279, 298, 300, 301 and 331.
Dependencies are inferred from the claim text.