Process for the purification of poliovirus from cell cultures
AssigneeJanssen Vaccines & Prevention B.V.
InventorsMariken Segers, Beckley Kungah Nfor, Feras Nachmi Alazi, Marcel Leo De Vocht
Life of this patent
Estimating the term…
Abstract
The disclosure provides methods for poliovirus purification from crude cell culture harvests using a detergent followed by a clarification step.
Claims11
A method of purifying poliovirus from a crude cell culture harvest containing poliovirus, said method comprising the steps of:
- a) adding a cationic detergent to the crude cell culture harvest to obtain a mixture;
- and
- b) clarifying a mixture obtained from step a) to obtain a clarified harvest with poliovirus particles;
- wherein the combination of step a) and step b) results in a reduction in host cell DNA of at least 4 log 10.
A method of enhancing poliovirus release from a crude cell culture harvest containing poliovirus, said method comprising the steps of:
- a) adding a cationic detergent to the crude cell culture harvest to obtain a mixture;
- and
- b) clarifying a mixture obtained from step a) to obtain a clarified harvest with poliovirus particles;
- wherein the combination of step a) and step b) results in a reduction in host cell DNA of at least 4 log 10.
A method of purifying poliovirus from a crude cell culture harvest containing poliovirus, said method comprising the steps of:
- a) adding a cationic detergent to the crude cell culture harvest to obtain a mixture;
- b) clarifying a mixture obtained from step a) to obtain a clarified harvest with poliovirus particles;
- and
- c) subjecting the clarified harvest obtained in step b) to a capture step to obtain a poliovirus-containing suspension;
- wherein the combination of step a) and step b) results in a reduction in host cell DNA of at least 4 log 10.
A method of purifying poliovirus from a crude cell culture harvest containing poliovirus, said method comprising the steps of:
- a) adding a cationic detergent to the crude cell culture harvest to obtain a mixture;
- b) clarifying a mixture obtained from step a) to obtain a clarified harvest with poliovirus particles;
- c) subjecting the clarified harvest obtained in step b) to a cationic exchange chromatography step to obtain a poliovirus-containing suspension;
- and
- d) further separating the poliovirus from the poliovirus-containing suspension by size exclusion chromatography;
- wherein the combination of step a) and step b) results in a reduction in host cell DNA of at least 4 log 10.
Dependencies are inferred from the claim text.