Fluid pumping and bioreactor system

A set for decellularizing tissue comprising:

• at least one reservoir including a plurality of ports, the plurality of ports including at least one mix port and at least one pump port;
• at least one bioreactor including at least one bioreactor port, the at least one bioreactor surrounding the tissue;
• at least one pump cassette, the pump cassette including at least one pump, at least one reservoir port fluidically connecting the at least one pump cassette to the at least one reservoir at the at least one pump port, the at least one pump cassette including at least one bioreactor interface port fluidically connecting the at least one pump cassette to the at least one bioreactor at the at least one bioreactor port, the at least one pump cassette including at least one first fluid bus and at least one pump cassette valve, the at least one pump cassette valve managing the routing of a first fluid from the at least one reservoir through the at least one pump to the at least one bioreactor interface port;
• at least one mix cassette including at least one dilution port, the at least one dilution port fluidically coupling the at least one mix cassette with a medium, the at least one mix cassette include at least one reservoir port fluidically coupling the at least one mix cassette with the at least one reservoir, the at least one mix cassette including at least one solution port fluidically coupling the at least one mix cassette with at least one second fluid, the at least one mix cassette including at least one mix pump, the at least one mix cassette including at least one second fluid bus, the at least one mix cassette including at least one mix cassette valve managing the routing of the at least one second fluid from the at least one solution port through the at least one mix pump to the at least one reservoir port, the at least one mix pump mixing together a plurality of the at least one second fluid;
• and
• tubing enabling the fluidic connections at least among the at least one first fluid bus and the at least one second fluid bus.