Preparation method, product and application of non-free radical photo-crosslinked hydrogel material

A preparation method of hydrogel by non-free radical photo-crosslinking reaction, comprising:

• obtaining solution A by dissolving component A in biocompatible medium, and obtaining solution B by dissolving component B that is a polymer derivative containing hydrazide, hydroxylamine or primary amine in biocompatible medium;
• obtaining a precursor solution of hydrogel by mixing solution A and solution B homogeneously;
• crosslinking the precursor solution to form a hydrogel under light irradiation via reacting aldehyde groups generated from o-nitrobenzyl of the component A after photo-activation with hydrazine, hydroxylamine, or primary amine groups of the component B, the reaction between the aldehyde groups and the hydrozin, hydroxylamine, or primary amine groups forming hydrazone, oxime and schiff base, respectively,
• wherein the component A is a derivative of a polymer modified with o-nitrobenzyl phototrigger as shown in structural formula I:
• where R1 is —H, ester bond selected from —CO(CH2)xCH3, —CO(CH2CH2O)xCH3, —CO(CH2)x(CH2CH2O)yCH3, ether bond selected from —(CH2)xCH3, —(CH2CH2O)xCH3, —(CH2)x(CH2CH2O)yCH3,
• carbonic ester bond selected from —COO(CH2)xCH3, —COO(CH2CH2O)xCH3, —COO(CH2)x(CH2CH2O)yCH3, or carbamate bond selected from —CONH(CH2)xCH3, —CONH(CH2CH2O)xCH3, —CONH(CH2)x(CH2CH2O)yCH3, where the x and y≥0 and are integer;
• R2 is —H or substituent group selected from —O(CH2)xCH3, —O(CH2CH2O)xCH3, —O(CH2)x(CH2CH2O)yCH3, where the x and y≥0 and are integer;
• R3 is selected from amino linkage bond —O(CH2)xCONH(CH2)yNH—, halogenated linkage bond —O(CH2)x— and carboxyl linkage bond —O(CH2)xCO—, wherein the ether bond end of R3 being connected with the benzene ring of the structural formula I and the other end of R3 being connected with P1, x and y≥1 and being integer;
• R4 is —H or —CONH(CH2)xCH3, where the x≥0 and being integer;
• P1 is hydrophilic or water-soluble natural polysaccharide, protein or polypeptide, or hydrophilic or water-soluble synthetic polymer,
• wherein the component B is a polymer derivative containing hydrazide, hydroxylamine, or primary amine, as shown in structural formula IIA, IIB, IIC, respectively:
• wherein n≥2, P2, P3, P4 each independently are hydrophilic or water-soluble natural polysaccharide, protein, polypeptide, or hydrophilic or water-soluble synthetic polymer.