Method for isolation of polysaccharides
AssigneeAlbert Bartlett & Sons (Airdrie) Limited
InventorsCharles Daniel Bavington, Claire Moss
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Abstract
A process for the isolation of modified polysaccharide product from potato, in particular a polysaccharide product that can be used as an immunomodulatory is described. More particularly, the extraction and modification of a rhamnogalacturonan I(RG-1) fragment product from a potato using a defined process, and the use of this product to provide immunomodulatory activity to a subject.
Claims15
A process for the preparation of a fragment of RG-1, wherein the process comprises the steps:
- providing RG-1 obtained from the enzymatic extraction of potato
- preparing a fragment of said RG-1, the fragment having an average molecular weight in the range 5 kDa to 30 kDa, by selective depolymerisation of said RG-1 to provide the fragment wherein the fragment has a monosaccharide composition comprising
- Arabinose 7-13%,
- Rhamnose 5-10%,
- Xylose 0-1%,
- Galacturonic acid 20-40% and
- Galactose 35-60%.
A fragment of RG-1 wherein the fragment has molecular weight of less than 30 KDa, which is capable of inhibiting neutrophil elastase and comprises a monosaccharide profile
- Monosaccharide
- composition
- %
- Arabinose
- 7-13
- Rhamnose
- 5-10
- Galacturonic acid
- 20-40
- Galactose
- 35-60
- Mannose
- 0-5
- Fucose
- n.d.
- Xylose
- 0-1
- Glucose
- 0-5
- Ratio GalA:
- Gal
- 0.57-0.66
- Ratio GalA:
- Rha
- 4.
A fragment obtained from a process comprising
- providing RG-1 obtained from the enzymatic extraction of potato
- preparing a fragment of said RG-1, the fragment having an average molecular weight in the range 5 kDa to 30 kDa, by selective depolymerisation of said RG-1 to provide the fragment wherein the fragment has a monosaccharide composition comprising
- Arabinose 7-13%,
- Rhamnose 5-10%
- Galacturonic acid 20-40% and
- Galactose 35-60%.
Dependencies are inferred from the claim text.