Synthetic antibodies to BAX and uses thereof
AssigneesAlbert Einstein College of Medicine, The Governing Council of the University of Toronto
InventorsEvripidis Gavathiotis, Jonathan R. Lai, Sachdev Sidhu
Life of this patent
Estimating the term…
Abstract
Synthetic fragment antigen-binding (Fab) antibodies are disclosed that bind to an N-terminal activation site of BCL-2-associated X-protein (BAX) and inhibit BAX activation. Also disclosed are methods of using the Fabs for measuring inactive monomeric BAX levels, screening for small molecules that bind to an N-terminal activation site of BAX, inhibiting apoptotic cell death, and predicting the ability of a cancer therapy to promote apoptotic cell death.
Claims9
A synthetic fragment antigen-binding (Fab) antibody that binds to an N-terminal activation site of BCL-2-associated X-protein (BAX) and inhibits BAX activation, wherein the synthetic fragment antigen-binding (Fab) antibody is selected from the group consisting of
- 2B1 Fab, having a light chain comprising a CDR L1, a CDRL2 and a CDRL3 region comprising the amino acid sequence SEQ ID NO:
- 1 (QYSGSGHYLI), a CDRH1 region comprising the amino sequence SEQ ID NO:
- 2 (IYSSSM), a CDRH2 region comprising the amino acid sequence SEQ ID NO:
- 3 (SISSSSSYTS) and a CDRH3 region comprising the amino acid sequence SEQ ID NO:
- 4 (RGYWYYWAWWASAMD);
- 3G11 Fab, having a light chain comprising a CDR L1, a CDRL2 and a CDRL3 region comprising the amino acid sequence SEQ ID NO:
- 9 (QWSFGPI), a CDRH1 region comprising the amino sequence SEQ ID NO:
- 10 (ISYYSM), a CDRH2 region comprising the amino acid sequence SEQ ID NO:
- 11(SIYPYSSSTY) and a CDRH3 region comprising the amino acid sequence SEQ ID NO:
- 12 (RSSAMD);
- and
- 2D9 Fab, having a light chain comprising a CDR L1, a CDRL2 and a CDRL3 region comprising the amino acid sequence SEQ ID NO:
- 13 (QWSHYLI), a CDRH1 region comprising the amino sequence SEQ ID NO:
- 14(LYYYSM), a CDRH2 region comprising the amino acid sequence SEQ ID NO:
- 15 (SISPSYGYTS) and a CDRH3 region comprising the amino acid sequence SEQ ID NO:
- 16 (RSSFYYYALD).
Dependencies are inferred from the claim text.