Method for preparing C1q recombinant protein
AssigneesCommissariat A L'energie Atomique Et Aux Energies Alternatives, Institut National De La Sante De La Recherche Medicale (Inserm)
InventorsNicole Thielens, Isabelle Bally
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Abstract
The present invention relates to a method for recombinant production of a C1q protein or a variant of the C1q protein, in which the protein is recovered from an in vitro culture of cells expressing a C1qA subunit or a variant of the C1qA subunit, a C1qB subunit or a variant of the C1qB subunit, and a C1qC subunit or a variant of the C1qC subunit, in which at least one of the subunits or subunit variants also has at the N-terminus or C-terminus a sequence of amino acids of at least six residues, at least 40% of which are glutamic acid and/or aspartic acid residues.
Claims5
A method for recombinantly producing a C1q protein, or a C1q protein variant, comprising:
- providing an in vitro culture of mammalian cells stably expressing a mature C1qA subunit having the sequence of SEQ ID NO:
- 1 or a sequence at least 80% identical to SEQ ID NO:
- 1 provided that it can form a C1q protein, a mature C1qB subunit having the sequence of SEQ ID NO:
- 3 or a sequence at least 80% identical to SEQ ID NO:
- 3 provided that it can form a C1q protein, and a mature C1qC subunit having the sequence of SEQ ID NO:
- 5 or a sequence at least 80% identical to SEQ ID NO:
- 5 provided that it can form a C1q protein, and in which at least one of the mature subunits also bears, at the C-terminal end, an additional amino acid sequence of DYKDDDDK (SEQ ID NO:
- 8) or of EQKLISEEDL (SEQ ID NO:
- 17);
- and
- recovering the recombinant C1q protein or C1q protein variant from the in vitro culture of cells by a process comprising at least one affinity purification step.
Dependencies are inferred from the claim text.