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US 10,294,283 B2Granted
Single-domain antibodies with improved thermal stability under cytoplasmic expression
AssigneeThe United States of America as represented by the Secretary of the Navy
InventorsDaniel Zabetakis, George P. Anderson, Ellen R. Goldman, Kendrick Turner, P. Audrey Brozozog Lee
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Abstract
Methods of producing a single-domain antibody (sdAb) include causing a bacteria to express the sdAb into cytoplasm of the bacteria, wherein the sdAb is expressed as a fusion protein with the acid tail of α-synuclein; and then purifying the sdAb, wherein the fusion protein is expressed free of a periplasmic location tag. Such antibodies have the unexpected ability to refold after thermal denaturation.
Claims8
A method of producing a single-domain antibody (sdAb), the method comprising:
- causing a bacteria to express the sdAb into cytoplasm of the bacteria, wherein the sdAb is expressed as a fusion protein with the acid tail of α-synuclein;
- and then
- purifying the sdAb,
- wherein the fusion protein is expressed free of a periplasmic location tag.
1 more element
Dependencies are inferred from the claim text.