Stabilized soluble pre-fusion RSV F polypeptides
AssigneeJanssen Vaccines & Prevention B.V.
InventorsJohannes P. M. Langedijk, Anders Krarup
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Abstract
This disclosure provides stable pre-fusion respiratory syncytial virus (RSV) F polypeptides, immunogenic compositions comprising the polypeptides, and uses thereof for the prevention and/or treatment of RSV infection.
Claims22
A recombinant respiratory syncytial virus (RSV) Fusion (F) polypeptide comprising, from amino-terminus to carboxyl-terminus, an F2 domain, a linking sequence consisting of SEQ ID NO:5, a truncated F1 domain, and a trimerization domain having the amino acid sequence of SEQ ID NO:4 linked to the truncated F1 domain at position 513 of the F polypeptide, wherein the truncated F1 domain comprises at least one mutation, as compared to wild-type F1 domain, selected from the group consisting of:
- (a) a mutation of the amino acid residue E at position 161 to P, Q or G;
- (b) a mutation of the amino acid residue S at position 173 to P;
- and
- (c) a mutation of the amino acid residue I at position 214 to P,
- wherein the numbering of amino acid residues is in accordance with that of SEQ ID NO:
- 1.
A recombinant respiratory syncytial virus (RSV) Fusion (F) polypeptide comprising, from amino-terminus to carboxyl-terminus, an F2 domain, a linking sequence consisting of SEQ ID NO:5, a truncated F1 domain, and a trimerization domain having the amino acid sequence of SEQ ID NO:4 linked to the truncated F1 domain at position 513 of the F polypeptide, wherein, as compared to wild-type F1 domain, the truncated F1 domain comprises a mutation of the amino acid residue D at position 486 to C and a mutation of the amino acid residue E at position 487 to C, wherein the numbering of amino acid residues is in accordance with that of SEQ ID NO:
- 1.
A recombinant respiratory syncytial virus (RSV) F polypeptide comprising an amino acid sequence selected from the group consisting of SEQ ID NO:
- 90-SEQ ID NO:
- 94.
Dependencies are inferred from the claim text.