Compound, nucleic acid, labeling substance, and detection method

A nucleic acid comprising at least one structure represented by the following chemical formula 1b-2 or (Ia-2):

• where in the chemical formulas 1b-2 and (Ia-2),
• R1 and R2 are each a hydrogen or a protecting group of an amino group and may be identical to or different from each other, or alternatively, R1 and R2 together may form a protecting group of an amino group, with the proviso that one of these substituents is always an acyl-type amine protecting group,
• R3 is a protecting group of a hydroxy group,
• J is a hydrogen atom, an alkyl group, or an alkoxy group,
• A is a hydrogen atom, a protected hydroxyl group, an alkyl group, an aralkyl group, an alkoxy group, a halogen, a trifluoromethyl group, a fluoroalkyl group, a trisubstituted silyl group, or a protected thiol group,
• or alternatively, J and A when bonded together may form a bridging linkage wherein J is CH2, O, or S, A is CH2, O, or S and J and A may be identical to or different from each other,
• 1, m, and n′ are each an integer of 2 to 100, 1, m, and n′ may be identical to or different from each other,
• Z11 and Z12 are each a fluorescent dye moiety that exhibits an exciton effect, and
• the mark “*” indicates a position at which the structure is bonded to a terminal PO331 group of a nucleic acid;
• a tautomer of the compound;
• or
• a salt of the compound, or the tautomer.

A nucleic acid comprising at least one structure represented by the following chemical formula (IIa-1) and includes a counter cation:

• where in the chemical formula (IIa-1),
• R1 and R2 are each a hydrogen or a protecting group of an amino group and may be identical to or different from each other, or alternatively, R1 and R2 together may form a protecting group of an amino group, with the proviso that one of these substituents is always an acyl-type amine protecting group,
• J is a hydrogen atom an alkyl group, or an alkoxy group,
• A is a protected hydroxyl group, an alkyl group, an aralkyl group, an alkoxy group, a halogen, a trifluoromethyl group, a fluoroalkyl group, a trisubstituted silyl group, or a protected thiol group,
• or alternatively, J and A when bonded together may form a bridging linkage wherein J is CH2, O, or S, A is CH2, O, or S and J and A may be identical to or different from each other,
• 1, m, and n′ are each an integer of 2 to 100,1, m, and n′ may be identical to or different from each other,
• Z11 and Z12 are each a fluorescent dye moiety that exhibits an exciton effect, and
• the mark “*” indicates a position at which the structure is bound to another atom or atomic group, and
• at least one O atom in a phosphoric acid linkage may be substituted with an S atom;
• a tautomer of the compound;
• or
• a salt of the compound, or the tautomer.

A nucleic acid comprising at least one structure represented by the following chemical formula 1b and includes one counter cation:

• where in the chemical formula 1b,
• R1 and R2 are each a hydrogen or a protecting group of an amino group and may be identical to or different from each other, or alternatively, R1 and R2 together may form a protecting group of an amino group, with the proviso that one of these substituents is always an acyl-type amine protecting group,
• J is a hydrogen atom , an alkyl group, or an alkoxy group,
• A is a hydrogen atom, a protected hydroxyl group, an alkyl group, an aralkyl group, an alkoxy group, a halogen, a trifluoromethyl group, a fluoroalkyl group, a trisubstituted silyl group, or a protected thiol group,
• or alternatively, J and A when bonded together may form a bridging linkage wherein J is CH2, O, or S, A is CH2, O, or S and J and A may be identical to or different from each other, and
• at least one O atom in a phosphoric acid linkage may be substituted with an S atom;
• a tautomer of the compound;
• or
• a salt of the compound, or the tautomer.

A nucleic acid comprising at least one structure represented by the following chemical formula 1c and includes one counter cation:

• where in the chemical formula 1c,
• R1 and R2 are each a hydrogen or a protecting group of an amino group and may be identical to or different from each other, or alternatively, R1 and R2 together may form a protecting group of an amino group, with the proviso that one of these substituents is always an acyl-type amine protecting group, and
• at least one O atom in a phosphoric acid linkage may be substituted with an S atom;
• a tautomer of the compound;
• or
• a salt of the compound, or the tautomer.