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US 10,294,258 B2Granted
Functionalization and purification of molecules by reversible group exchange
AssigneeIllumina, Inc.
InventorsFrank J. Steemers, Kevin L. Gunderson, Kerri York, Ryan Christopher Smith
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Abstract
Embodiments of the present disclosure include methods and compositions for functionalizing molecules, such as oligonucleotides, with functional groups, including polyhistidine tags useful in affinity methods. Some embodiments include methods for modifying and purifying complex mixtures of molecules by exchange of functional tags.
Claims16
A method for modifying a nucleic acid, the method comprising:
- (a) contacting a sample comprising a nucleic acid with a binding partner immobilized on an affinity substrate, wherein the nucleic acid comprises a purification tag, under conditions to form a first covalent bond coupling the nucleic acid to the binding partner;
- (b) separating the substrate-coupled nucleic acid from the sample, thereby isolating the nucleic acid from the sample;
- and
- (c) performing an exchange reaction comprising contacting the nucleic acid isolated in step (b) with an exchange reagent under conditions sufficient to break the first covalent bond between the nucleic acid and the binding partner and to form a second covalent bond between the exchange reagent and the nucleic acid.
1 more element
Dependencies are inferred from the claim text.