Test substance administration system for animal experiment

A test substance application system comprising:

• a respiration monitoring device that monitors a respiration state of an experimental animal detected by a respiration pick-up device, thereby measuring a timing upon switching from an expiratory phase to an inhalatory phase, and outputs a trigger signal at the timing,
• an applicator that is adapted for supplying and actively spraying a predetermined amount of the test substance via a nozzle into a nasal cavity or an oral cavity of the experimental animal when the trigger signal is outputted, wherein the test substance is sprayed as a powder in pressurized air,
• wherein the applicator is mounted to the nozzle, and
• wherein a longitudinal axis of the nozzle and a longitudinal axis of the applicator align with each other, forming a straight path from the applicator to the nasal cavity or the oral cavity of the experimental animal,
• an air supply mechanism that supplies the pressurized air only when the trigger signal is outputted, and
• two check valves connected, each by its own tube, to the nozzle, wherein the tubes are aligned perpendicular to the nozzle and in parallel to each other,
• wherein the only source of the pressurized air in the test substance application system is the air supply mechanism, and wherein the respiration pick-up device is directly attached to the nozzle.