Ready-to-use, hydrophilic, self-dispersive, fragmentable and biodegradable porous sponge matrix and a method of manufacturing thereof

A method of manufacturing a biodegradable porous sponge matrix, comprising the steps of:

• preparing a homogenous solution of at least two polymers by adding a first polymer to water to form a first polymer solution, and adding a second polymer to the first polymer solution to form a homogenous solution of the at least two polymers;
• wherein the step of preparing the homogenous solution of the at least two polymers further comprises dissolving of the at least two polymers to form the homogenous solution, wherein the step of dissolving comprises stirring of the at least two polymers at a temperature between 18-25° C., and at a relative humidity of 50-60% for between 20-30 minutes using a stirrer with an aspect ratio of the diameter of the container between 1.17 to 1.57 and between a rate of 1000-3600 rpm;
• introducing glacial acetic acid at a concentration between 0.5-2.5% w/v at rate of about 1 ml/minute and at a temperature between 18-25° C., and a relative humidity between 50-60% for between 5-10 minutes;
• introducing chitosan at a concentration between 0.5% to 1.2% w/v at the rate of about 1 mg/ml and at a temperature between 18-25° C., and a relative humidity between 50-60% for between 30-40 minutes;
• allowing the homogenous solution to freeze dry, thereby obtaining a porous sponge matrix;
• and
• wherein each polymer is selected from the group consisting of:
• gelatin, chitosan, collagen, alginate, polyvinyl alcohol, poly(vinyl pyrrolidone), polyurethane, polyethylene glycol, polypropylene glycol keratin, hyaluronic acid, carboxymethyl cellulose, gelatin hydrolysate, chitosan hydrolysate, partially denatured collagen and mucilaginous polysaccharides.