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US 10,293,060 B2Granted
Method for increasing expression of RNA-encoded proteins
AssigneeCureVac AG
InventorPatrick Baumhof
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AssigneeCureVac AG
InventorPatrick Baumhof
Estimating the term…
The invention relates to an RNA comprising at least one open reading frame (ORF) and comprising at least one modification, which increases the expression of the encoded peptide or protein. Furthermore, the invention relates to the medical use of such a modified RNA administered to a subject by jet injection. The invention relates further to a pharmaceutical composition and to a kit of parts comprising said modified RNA for administration by jet injection, preferably for use in the field of gene therapy and/or genetic vaccination. Additionally, the invention relates to a method for enhancing the (localized) expression of RNA-encoded peptides or proteins in the dermis or muscle (of a mammal) comprising administering the modified RNA by jet injection. And finally, the invention relates to a method of treatment comprising administering the modified RNA by jet injection to a subject in need thereof.
A method for enhancing the localized expression of an RNA-encoded polypeptide in the dermis or muscle of a mammal comprising administering by jet injection a modified RNA comprising an increased G/C content of a polypeptide coding region of the modified RNA as compared with the G/C content of the corresponding polypeptide coding region of the native RNA, wherein the polypeptide sequence encoded by the modified RNA is not altered compared with the polypeptide sequence encoded by the native RNA, the increased G/C content resulting in increased expression of the RNA-encoded polypeptide, wherein the RNA comprises at least one histone stem-loop.
Dependencies are inferred from the claim text.