Gene therapy to prevent reactions to allergens

A method of inhibiting or reducing an immune response or allergic reaction to an allergen in a mammal, comprising administering an AAV vector to the mammal,

• wherein the AAV vector comprises a promoter operably linked to a nucleic acid sequence that encodes an anti-IgE antibody or antigen binding fragment thereof,
• wherein the anti-IgE antibody or antigen binding fragment thereof comprises a heavy chain polypeptide and a light chain polypeptide,
• wherein the heavy chain polypeptide comprises three complementarity determining regions (CDRs), wherein CDR-H1 comprises the nucleic acid sequence of SEQ ID NO:
• 1, CDR-H2 comprises the nucleic acid sequence of SEQ ID NO:
• 2, and CDR-H3 comprises the nucleic acid sequence of SEQ ID NO:
• 3;
• and
• wherein the light chain polypeptide comprises three CDRs, wherein CDR-L1 comprises the nucleic acid sequence of SEQ ID NO:
• 4, CDR-L2 comprises the nucleic acid sequence of SEQ ID NO:
• 5, and CDR-L3 comprises the nucleic acid sequence of SEQ ID NO:
• 6,
• whereupon the nucleic acid is expressed and an immune response or allergic reaction against the allergen is inhibited or reduced.