Pharmaceutical composition for treating cancer and a method thereof

A method of treating cancer in a subject in need thereof, the method comprising:

• administering an effective amount of a compound of Formula (1) or Formula (2), a derivative thereof, a solvate thereof, or a combination thereof to the subject, wherein Formula (1) and Formula (2) are:
• where R1 is a hydrogen, an optionally substituted alkyl group, an optionally substituted cycloalkyl, an optionally substituted arylalkyl group, an optionally substituted alkanoyl, an optionally substituted aroyl, an optionally substituted alkylsulfonyl, or an optionally substituted arylsulfonyl;
• R2 is a carboxy group, an optionally substituted alkanoyloxy, an optionally substituted aroyloxy, or an optionally substituted carbamyl;
• R3 is O or an optionally substituted CH2;
• R4 is a pair of electrons, an optionally substituted alkyl group, an optionally substituted cycloalkyl, or an optionally substituted arylalkyl group, with the proviso when R4 is an optionally substituted alkyl group, an optionally substituted cycloalkyl, or an optionally substituted arylalkyl group, the nitrogen atom attached to R4 has a positive charge;
• and
• R5 and R6 are independently a hydrogen, a halogen, or a hydroxy.

A method of extracting a compound from Rhazya Stricta, the method comprising:

• mixing at least a first part of Rhazya Stricta or a first extract of Rhazya Stricta with a solution comprising water and an acid thereby forming a second extract;
• mixing the second extract with an organic solvent thereby forming a first aqueous layer and a first organic layer;
• mixing the first aqueous layer with a base thereby forming a neutralized aqueous layer;
• mixing the neutralized aqueous layer with the organic solvent thereby forming a second aqueous layer and a second organic layer;
• concentrating the second organic layer thereby forming a residue comprising the compound;
• and
• isolating the compound, wherein the compound is: