Liposomal compositions and uses of same
AssigneeTechnion Research & Development Foundation Limited
InventorsMarcelle Machluf, Tomer Bronshtein
Life of this patent
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Abstract
Compositions comprising liposomes composed of whole cell membrane fraction are provided. The liposomes may be attached to, or encapsulate a pharmaceutical agent. Also provided are methods of generating and using these liposomes.
Claims16
A composition-of matter comprising a liposome composed of a whole cell membrane fraction, wherein the liposome exhibits native membrane symmetry and expression of native markers, said liposome encapsulating or conjugated to a pharmaceutical agent being exogenous to the cell from which said whole cell membrane is obtained and said liposome being devoid of the cytoplasmic content of said cell.
A composition-of matter comprising a liposome composed of a whole cell membrane fraction of a cell, wherein said liposome exhibits native membrane symmetry and expression of native markers and wherein said liposome is devoid of the cytoplasmic content of said cell.
A method of producing liposomes exhibiting native membrane symmetry and expression of native markers, the method comprising,
- (a) subjecting cells to hypotonic conditions, so as to obtain ruptured cell membranes and/or ghosts;
- and
- (b) homogenizing said ruptured cell membranes and/or ghosts to thereby produce liposomes, wherein the method is devoid of membrane solubilizaton in the presence of a detergent,
- thereby producing the liposomes exhibiting native membrane symmetry and expression of native markers.
Dependencies are inferred from the claim text.