Back to results
US 10,292,592 B2Granted
Method and apparatus for optical recording of biological parameters in freely moving animals
AssigneeThe Board of Trustees of the Leland Stanford Junior University
InventorsJesse D. Marshall, Mark J. Schnitzer
Life of this patent
Estimating the term…
Abstract
A system and method for monitoring biological parameters in freely moving animals that, in the illustrative embodiment, comprises a two-color optical measurement/recording system that is combined with a fluorescent protein reporter of cellular membrane potentials and a fluorescent protein that is insensitive to such cellular membrane potentials. The two wavelengths are used to un-mix physiological artifacts in recordings that occur in freely moving animals.
Claims20
A method comprising:
- delivering, to a target tissue, excitation light having a first wavelength and a second wavelength, wherein the target tissue contains a sensor and a reference;
- collecting optical domain responses from the sensor and the reference, the response from the sensor includes information pertaining to a biological parameter of interest as well as information pertaining to physiological artifacts and the response from the reference includes information pertaining to the physiological artifacts but not the biological parameter;
- and
- extracting the information pertaining to the biological parameter free of physiological artifacts.
1 more element
A system comprising:
- an excitation light subsystem, wherein the excitation light subsystem generates light having a first wavelength and a second wavelength, wherein the first wavelength is suitable for exciting fluorescence in a sensor, but not a reference, and the second wavelength is suitable for exciting fluorescence in the reference but not the sensor, and wherein fluorescence from the sensor contains information pertaining to a biological parameter of interest and physiological artifacts, including hemodynamic artifacts, and fluorescence from the reference contains information pertaining to the physiological artifacts, including hemodynamic artifacts, but not the biological parameter of interest;
- a waveguide for propagating the light to a target tissue and receiving fluorescence from the sensor and the reference;
- a signal processing subsystem comprising a processor that:
- (1) generates an estimate of hemodynamic artifacts;
- (2) extracts information pertaining to the biological parameter of interest free of any physiological artifacts by generating a linear unmixing matrix using the estimate of hemodynamic artifacts as an input.
2 more elements
A method comprising:
- delivering a fluorescent sensor protein to a target tissue, wherein the sensor protein is responsive to a membrane voltage, hemodynamic artifacts, and motion artifacts;
- delivering a fluorescent reference protein to the target tissue, wherein the reference protein is responsive to the hemodynamic artifacts and the motion artifacts but not to membrane voltage;
- delivering excitation light to the target tissue to cause the sensor protein and the reference protein to fluoresce;
- collecting the fluorescence from the sensor protein and the reference protein;
- separating the fluorescence from the sensor protein and the reference protein;
- converting the fluorescence from the sensor protein to a first electrical signal and the fluorescence for the reference protein to a second electrical signal;
- estimating the hemodynamic artifacts present in the first electrical signal;
- and
- extracting a signal indicative of membrane voltage, free of hemodynamic and motion artifacts, using the estimate of hemodynamic artifacts and independent component analysis.
6 more elements
Dependencies are inferred from the claim text.