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US 10,292,382 B2Granted
No-spin cryopreservation technique and resulting products
AssigneeBiopredic International Sarl
InventorsBelkacem Bouaita, Sandrine Camus, Nathalie Rougier, Ruo Ya Li, Christophe Chesne, David M. Steen
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Abstract
Methods and processes for cryopreservation and direct cell thawing and seeding or suspension after cryopreservation, including methods that eliminate the necessity of post-thaw wash, spin, and frequent practice of performing a cell count. Cell compositions and no-spin cell products produced using the methods are also described.
Claims16
A method for thawing and recovery of cryopreserved hepatocytes, said method comprising:
- providing a concentrated composition of cryopreserved hepatocytes comprising a first amount of hepatocytes dispersed in a freezing solution, said freezing solution comprising a cryoprotectant dispersed in medium;
- thawing said concentrated composition of cryopreserved hepatocytes to yield a thawed composition of hepatocytes, wherein said thawed composition of hepatocytes comprises thawed hepatocytes and cryoprotectant dispersed in a medium;
- and
- directly plating said thawed composition of hepatocytes or directly suspending said thawed composition of hepatocytes in a suspension assay, without removal of said cryoprotectant.
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Dependencies are inferred from the claim text.