Indole-derived compound production
AssigneeBoard of Trustees of Southern Illinois University
InventorAldwin Anterola
Life of this patent
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Abstract
Among the various aspects of the present disclosure is the provision of a transgenic organism, an artificial DNA construct, and methods for producing a transgenic organism for indigo, indirubin, and other indole-derived compound production. Another aspect of the present disclosure is the provision of a transgenic organism wherein the indole-derived compound imparts color to the transgenic organism or to a portion of the transgenic organism.
Claims19
A transgenic organism engineered to accumulate an indole-derived compound, the organism transformed with an artificial DNA construct comprising, as operably associated components in the 5′ to 3′ direction of transcription:
- (i) a promoter that functions in the organism;
- (ii) a first transcribable nucleic acid sequence encoding a cytochrome P450 polypeptide, comprising:
- (1) SEQ ID NO 3 (ItB4), or a nucleotide sequence at least 95% identical thereto and encoding a polypeptide catalyzing formation of 2-hydroxyindole from indole;
- or
- (2) SEQ ID NO:
- 4 (ItB24), or a nucleotide sequence at least 95% identical thereto and encoding a polypeptide catalyzing formation of 3-hydroxyindole from indole;
- and
- (iii) a transcriptional termination sequence;
- wherein the organism produces increased levels of indole-derived compounds, or precursors thereof, compared to an organism without the artificial DNA construct.
A method for producing a transgenic organism comprising:
- transforming an organism with an artificial DNA construct, the artificial construct comprising, as operably associated components in the 5′ to 3′ direction of transcription,
- (i) a promoter that functions in the organism;
- (ii) a first transcribable nucleic acid sequence encoding a cytochrome P450 polypeptide, comprising:
- (1) SEQ ID NO 3 (ItB4), or a nucleotide sequence at least 95% identical thereto and encoding a polypeptide catalyzing formation of 2-hydroxyindole from indole;
- or
- (2) SEQ ID NO:
- 4 (ItB24), or a nucleotide sequence at least 95% identical thereto and encoding a polypeptide catalyzing formation of 3-hydroxyindole from indole;
- and
- (iii) a transcriptional termination sequence;
- wherein the organism produces increased levels of indole-derived compounds, or precursors thereof, compared to an organism without the artificial DNA construct.
An artificial DNA construct comprising:
- (i) a promoter that functions in the organism;
- (ii) a first transcribable nucleic acid sequence encoding a cytochrome P450 polypeptide, comprising
- (1) SEQ ID NO 3 (ItB4), or a nucleotide sequence at least 95% identical thereto and encoding a polypeptide catalyzing formation of 2-hydroxyindole from indole;
- or
- (2) SEQ ID NO:
- 4 (ItB24), or a nucleotide sequence at least 95% identical thereto and encoding a polypeptide catalyzing formation of 3-hydroxyindole from indole;
- and
- (iii) a transcriptional termination sequence.
Dependencies are inferred from the claim text.