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US 10,287,568 B2Granted
Methods for extracting and purifying non-denatured proteins
AssigneeImpossible Foods Inc.
InventorsAniket Kale, Ranjani Varadan, Simon Christopher Davis
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Abstract
Materials and methods for extracting and purifying proteins are provided. For example, the materials and methods provided herein can be used for extracting and purifying proteins that denature at low temperature.
Claims10
A method for purifying a protein from a biomass, comprising:
- extracting the biomass with an aqueous solution and, optionally, a flocculant, to generate an extraction slurry that contains bulk solids and an extract;
- removing solids from the extraction slurry;
- adding a hydrophilic polymer to the extraction slurry, wherein the hydrophilic polymer is immobilized, and wherein the hydrophilic polymer comprises polyethylene glycol (PEG), butylene glycol, hexylene glycol, glycerin, diglycerin, diethylene glycol, dipropylene glycol, or a mixture thereof;
- and
- separating protein from the immobilized hydrophilic polymer to generate a product phase that contains the protein.
2 more elements
Dependencies are inferred from the claim text.