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US 10,287,553 B2Granted
In vitro method for culturing stem cells
AssigneeAgency for Science, Technology and Research
InventorsDaniele Zink, Ming Ni, Karthikeyan Narayanan, Karthikeyan Kandasamy, Andrew C. A. Wan, Jackie Y. Ying
Life of this patent
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Abstract
There is provided a method for culturing a stem cell in vitro. The method comprises providing a substrate surface coated with a coating comprising a molecule having a catechol moiety or a polymer thereof; and growing a stem cell on said coated substrate surface in a growth medium.
Claims14
An in vitro method for expanding a pluripotent stem cell by self-renewal while maintaining the level of differentiation of the stem cell, the method comprising:
- providing a substrate surface coated with a coating comprising L-3,4-dihydroxy-phenylalanine (L-DOPA);
- and
- growing a pluripotent stem cell attached on said coated substrate surface in a growth medium under growth conditions that do not induce differentiation of the stem cell for at least 10 passages.
Dependencies are inferred from the claim text.