Chimeric antigen receptors targeting CD-19
AssigneeThe United States of America, as represented by the Secretary, Department of Health and Human Services
InventorJames N. Kochenderfer
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Abstract
The invention is directed to a chimeric antigen receptor (CAR) directed against CD19, which comprises an amino acid sequence of any one of SEQ ID NO: 1-SEQ ID NO: 13. The invention also provides T-cells expressing the CAR and methods for destroying malignant B-cells.
Claims18
A chimeric antigen receptor (CAR) directed against CD19, which comprises an amino acid sequence of SEQ ID) NO:
- 1, SEQ ID) NO:
- 2, SEQ ID NO:
- 3, SEQ ID NO:
- 4, SEQ ID NO:
- 5, SEQ ID NO:
- 6, SEQ ID NO:
- 7, SEQ ID NO:
- 8, SEQ ID NO:
- 9, SEQ ID NO:
- 10, SEQ ID NO:
- 11, SEQ ID NO:
- 12, or SEQ ID NO:
- 13.
A nucleic acid encoding a chimeric antigen receptor(CAR) directed against CD19, the CAR comprises an amino acid sequence of SEQ ID) NO:
- 1, SEQ ID) NO:
- 2, SEQ ID NO:
- 3, SEQ ID NO:
- 4, SEQ ID NO:
- 5, SEQ ID NO:
- 6, SEQ ID NO:
- 7, SEQ ID NO:
- 8, SEQ ID NO:
- 9, SEQ ID NO:
- 10, SEQ ID NO:
- 11, SEQ ID NO:
- 12, or SEQ ID NO:
- 13.
A chimeric antigen receptor (CAR) comprising the amino acid sequence of each of the following that are present in SEQ ID NO:
- 4 or SEQ ID NO:
- 9;
- (i) the extracellular spacer,
- (ii) the transmembrane domain derived from a human CD8α molecule, and
- (iii) the intracellular T-cell signaling domains derived from
- (a) one or both of a human CD28 molecule and a human CD27 molecule, and
- (b) a human CD3 ζmolecule.
A chimeric antigen receptor (CAR) comprising the amino acid sequence of each of the following that are present in SEQ ID NO:
- 10 or SEQ ID NO:
- 11;
- (i) the extracellular spacer,
- (ii) the transmembrane domain derived from a human CD8α , molecule, and
- (iii) the intracellular T-cell signaling domains derived from
- (a) one or both of a human CD28 molecule and a human CD27 molecule, and
- (b) the gamma chain of FcεRI.
A chimeric antigen receptor (CAR) comprising the amino acid sequence of each of the following that are present in SEQ ID NO:
- 12 or SEQ ID NO:
- 13;
- (i) the extracellular spacer,
- (ii) the transmembrane domain derived from a human CD8α molecule, and
- (iii) the intracellular T-cell signaling domains derived from a human CD28molecule and the gamma chain of FcεRI.
A method of making a cell expressing a chimeric antigen receptor (CAR), the method comprising introducing a vector encoding the CAR of chum 12 into a T-cell or NK cell.
Dependencies are inferred from the claim text.