Mitochondrial proteins constructs and uses thereof
AssigneeYissum Research Development Company of the Hebrew University of Jerusalem
InventorsHaya Lorberboum-Galski, Hagar Greif
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Abstract
Disclosed are fusion protein constructs comprising a functional mitochondrial protein, that can enter mitochondria within intact cells. Further disclosed are methods of treating mitochondrial disorders by the disclosed fusion proteins and compositions therefor.
Claims7
A fusion protein comprising an HIV-1 transactivator of transcription (TAT) domain fused to human ornithine transcarbamoylase (OTC) and a mitochondria targeting sequence (MTS) of human citrate synthase situated between said TAT domain and said OTC, wherein said OTC is C-terminal to said MTS of human citrate synthase.
A fusion protein having the amino acid sequence denoted by SEQ ID NO:
- 49, comprising a HIV-1 transactivator of transcription (TAT) domain having the amino acid sequence denoted by SEQ ID NO:
- 27 fused to human ornithine transcarbamoylase (OTC) having the amino acid sequence denoted by SEQ ID NO:
- 39 and a mitochondria targeting sequence (MTS) of human citrate synthase having the amino acid sequence denoted by SEQ ID NO:
- 23, said MTS situated between said TAT domain and said OTC, and is linked to said TAT domain via a linker having the amino acid sequence denoted by SEQ ID NO:
- 32, and wherein said OTC is C-terminal to said MTS of human citrate synthase.
Dependencies are inferred from the claim text.