Protein biosensors, cross reactive sensor arrays and methods of use thereof
AssigneeYeda Research and Development Co. Ltd.
InventorsDavid Margulies, Leila Motiei, Zohar Pode
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Abstract
The present invention is directed to fluorescent protein biosensors based on oligonucleotide cross reactive sensor arrays including a selective and a non-selective protein surface binding domain for protein detection. Interaction of different protein isoforms with the sensors of this invention yields a unique optical signature for each protein. The unique optical signature allows differentiating between closely related protein isoforms and diagnosing diseases and disorders associated with the proteins also in biofluids.
Claims36
An oligodeoxyribose nucleotide (ODN) derivatives duplex, which consists of
- a first ODN derivative comprising a first ODN sequence, at least one selective protein binder and a donor or acceptor;
- and
- a second ODN derivative comprising a second ODN sequence, which is complementary to said first ODN sequence, at least one non-selective protein surface binder and a donor or acceptor;
- wherein if one ODN derivative comprises a donor, the other one comprises an acceptor,
- at least one of the donor or acceptor is a fluorophore;
- and
- wherein said non-selective protein surface binder comprises a peptide tripod, represented by the structure of formula A:
A protein sensor, said sensor comprises a non-selective protein surface binder represented by the structure of formula A:
Dependencies are inferred from the claim text.