Chromogen layering for color generation
AssigneesDiagnostic BioSystems, Marc Key
InventorsBipin Gupta, Marc Key
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Abstract
The present disclosure relates methods of chromogen layering, wherein a first chromogen and a first stain (color) are produced on a sample, specific for a first analyte, followed by a second chromogen and a second stain (color) being produced on the same sample, specific for a second analyte. In addition, if desired, by overlaying the second stain on top of the first stain, a unique third color is produced that is specific for a third analyte. Therefore, the distribution of different colors throughout the sample could be used to identify at least two or more analytes simultaneously within a single sample.
Claims9
A method of staining a biological specimen for a first analyte, the method comprising:
- obtaining a section of a biological specimen;
- contacting the section with a first antibody directed towards the first analyte, wherein the first antibody is labeled with a first marker or wherein the first antibody becomes labeled with a first marker by means of a linking step;
- contacting the section with a first chromogen that reacts with the first marker to generate a first color;
- contacting the section with a second chromogen that reacts with the first marker directed to the first analyte to generate a second color which overlays the first color creating a third color;
- and
- visually observing the presence or absence of the first, the second, or the third color wherein the third color is created by overlaying the first and the second colors onto the first marker targeted to the first analyte.
Dependencies are inferred from the claim text.