Host cells and methods for producing cinnamoyl anthranilate and analogs thereof

A culture medium comprising:

• (A) a genetically modified host cell comprising (a) an hydroxycinnamoyl/benzoyl-CoA:anthranilate N-hydroxycinnamoyl/benzoyltransferase (HCBT, EC 2.3.1.144) having an amino acid sequence at least 70% identical to SEQ ID NO:1 capable of catalyzing the formation of a cinnamoyl anthranilate, or analog thereof, from a cinnamoyl-CoA, or analog thereof, and an anthranilate, or analog thereof, and (b) a 4-coumarate:CoA ligase (4CL, EC 6.2.1.12) having an amino acid sequence at least 70% identical to SEQ ID NO:3 capable of catalyzing the formation of a cinnamic acid, or analog thereof, into a corresponding cinnamoyl-CoA thioester, or analog thereof;
• and (B) a compound having the following chemical structure:
• wherein R1 and R2 are each H;
• R3 is OCH3;
• and R4 is OCH3.