Genetically engineered plants with increased vegetative oil content
AssigneeBoard of Trustees of Michigan State University
InventorsChristoph Benning, Sanjaya
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Abstract
The invention relates to genetically modified agricultural plants with increased oil content in vegetative tissues, as well as to expression systems, plant cells, seeds and vegetative tissues related thereto.
Claims31
A method of increasing oil content in vegetative tissues of a plant, comprising genetically modifying the plant to comprise a plant expression system, or inducing expression of a plant expression system, wherein the plant expression system comprises a combination of:
- (a) a first expression cassette comprising a nucleic acid segment encoding a WRINKLED1 transcription factor;
- and
- (b) a second expression cassette comprising a nucleic acid segment encoding an inhibitory nucleic acid comprising a nucleic acid with at least 95% sequence identity to at least 20 nucleotides of the nucleotide sequence encoding an ADP-glucose pyrophosphorylase with sequence SEQ ID NO:
- 5 or 75;
- wherein the plants modified to contain or induced to express the plant expression system produce at least 10-fold more oil in vegetative tissues than a plant of the same species that has not been modified to contain the plant expression system.
A plant expression system comprising:
- (a) a first expression cassette comprising a nucleic acid segment encoding a WRINKLED1 transcription factor;
- and
- (b) a second expression cassette comprising a nucleic acid segment encoding an inhibitory nucleic acid comprising a nucleic acid with at least 95% sequence identity to at least 20 nucleotides of the nucleotide sequence encoding an ADP-glucose pyrophosphorylase with sequence SEQ ID NO:
- 5 or 75;
- wherein the plant expression system, produces at least 10-fold more oil in plant vegetative tissues compared to plant vegetative tissues that have not been modified to contain the plant expression system.
A plant comprising a plant expression system that comprises a combination of:
- (a) a first expression cassette comprising a nucleic acid segment encoding a WRINKLED1 transcription factor;
- and
- (b) a second expression cassette comprising a nucleic acid segment encoding an inhibitory nucleic acid comprising a nucleic acid with at least 95% sequence identity to at least 20 nucleotides of the nucleotide sequence encoding an ADP-glucose pyrophosphorylase with sequence SEQ ID NO:
- 5 or 75;
- wherein the plant is a food plant, vegetable oil plant, forage plant, or fodder plant, and the plant's vegetative tissues have at least 10-fold more oil than a plant of the same species that does not contain the plant expression system.
A seed comprising a plant expression system that comprises a combination of:
- (a) a first expression cassette comprising a nucleic acid segment encoding a WRINKLED1 transcription factor;
- and
- (b) a second expression cassette comprising a nucleic acid segment encoding an inhibitory nucleic acid comprising a nucleic acid with at least 95% sequence identity to at least 20 nucleotides of the nucleotide sequence encoding an ADP-glucose pyrophosphorylase with sequence SEQ ID NO:
- 5 or 75;
- wherein the seed develops into a food plant, vegetable oil plant, forage plant, or fodder plant with vegetative tissues that have at least 10-fold more oil than a plant developed from a seed of the same species that does not contain the plant expression system.
Dependencies are inferred from the claim text.