US 10,274,488 B2Granted

Glycan arrays on PTFE-like aluminum coated glass slides and related methods

AssigneeAcademia Sinica

InventorsChi-Huey Wong, Chung-Yi Wu, Susan Y. Tseng

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Abstract

Aluminum coated glass slides provide a novel glycan array platform. Specifically, aluminum coated glass slides increase sensitivity of fluorescent based assay methods. Additionally, aluminum coated glass slides allows for mass spectroscopic analysis of carbohydrates and provide a platform for examining activity of cellulases. The unique properties of ACG slides include: 1) the metal oxide layer on the surface can be activated for grafting organic compounds such as modified oligosaccharides; 2) the surface remains electrically conductive, and the grafted oligosaccharides can be simultaneously characterized by mass spectrometry and carbohydrate-binding assay; and 3) the slides are more sensitive than transparent glass slides in binding analysis.

Claims8

Dependent claims

A method for fabricating an array of carbohydrates immobilized directly on an aluminum oxide surface of a single aluminum-coated glass slide, the method comprising:

(a) immobilizing a plurality of carbohydrates at discrete locations directly and covalently bound to a single underivatized surface of an aluminum-coated glass slide,
wherein the single aluminum-coated glass slide is conductive or semi-conductive of an electrical field,
wherein the single aluminum-coated glass slide comprises an aluminum oxide surface, and each carbohydrate of the plurality of carbohydrates in the array comprises a phosphonic acid functional group, such that each carbohydrate of the plurality of carbohydrates is a phosphonic acid-derivatized carbohydrate, such that each carbohydrate of the plurality of carbohydrates in the array is immobilized by a covalent bond to said aluminum oxide surface, and
wherein the single aluminum-coated glass slide comprises an aluminum oxide layer configured to be in contact with an aluminum layer, which is configured to be in contact with a single glass slide.

1 more element

Dependencies are inferred from the claim text.

Facts

Application: 14/182,290Filed 18 Feb 2014 · division
Priority: 15 Jul 2008
Published: US 2017/0038378 A19 Feb 2017
Granted: 30 Apr 2019
Term ends: Not estimatedThe source records don’t support an estimate.

Classification10 codes

G01N 33/553
The carrier being inorganic: metal or metal coated
C40B 40/12
Libraries containing only organic compounds: libraries containing saccharides or polysaccharides, or derivatives thereof
C40B 50/14
Methods of creating libraries, e.g. combinatorial synthesis: solid phase synthesis, i.e. wherein one or more library building blocks are bound to…
G01N 33/582
Involving labelled substances: with fluorescent label

Family

Application61/080,931Us provisional application · filed 15 Jul 2008
Application61/107,624Us provisional application · filed 22 Oct 2008
Application12/503,797Parent of this division · filed 15 Jul 2009
US 2017/0038378 A1Pre-grant publication · published 9 Feb 2017
US 8,680,020 B2Parent of this division
US 2010/0016171 A1Published application
US 10,274,488 B2This patent

From the US filing history. Foreign family members aren’t listed.