Plants with improved photosynthetic carbon fixation capacity
AssigneeBASF SE
InventorsChristoph Peterhaensel, Jeroen Van Rie, Claus Frohberg
Life of this patent
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Abstract
Plants are provided with increased ribulose-1,5-bisphosphate (RuBP) regeneration capacity during the Calvin cycle through increased expression of sedoheptulose 1,7 bisphosphatase, in combination with reduced photo-respiratory losses through expression of glycolate catabolizing enzymes. Such plants have a greater growth rate and/or improved biomass and/or increased carbon fixation compared to untreated plants, or plants comprising only one of the features above.
Claims33
A method for improving the assimilation of carbon in a plant comprising the steps of:
- a. inserting into the genome of a plant a nucleic acid encoding an enzyme comprising sedoheptulose 1,7-bisphosphatase activity operably linked to a plant-expressible promoter, and optionally to a 3′ end region involved in transcription termination and/or polyadenylation, wherein said enzyme comprising sedoheptulose 1,7-bisphosphatase activity comprises an amino acid sequence having at least 95% identity to the amino acid sequence of SEQ ID No. 2;
- and
- b. inserting into the genome of said plant one or more nucleic acids encoding one or more polypeptides having the enzymatic activity of glycolate dehydrogenase operably linked to a plant-expressible promoter, and optionally to a 3′ end region involved in transcription termination and/or polyadenylation, wherein said one or more polypeptides are located in chloroplasts of said plants.
A plant cell, a plant part, a plant or a seed comprising in its genome a nucleic acid encoding an enzyme comprising sedoheptulose 1,7-bisphosphatase activity operably linked to a plant-expressible promoter, and optionally to a 3′ end region involved in transcription termination and/or polyadenylation;
- and one or more nucleic acids encoding one or more polypeptides having the enzymatic activity of glycolate dehydrogenase operably linked to a plant-expressible promoter, and optionally to a 3′ end region involved in transcription termination and/or polyadenylation, wherein said enzyme comprising sedoheptulose 1,7-bisphosphatase activity comprises an amino acid sequence having at least 95% identity to the amino acid sequence of SEQ ID No. 2, and wherein said one or more polypeptides are located in chloroplasts of said plants.
A method for improving the assimilation of carbon in a plant or for increasing the growth rate or improving the biomass production of a plant, said method comprising the steps of inserting into the genome of a plant
- a. a nucleic acid encoding an enzyme comprising sedoheptulose 1,7-bisphosphatase activity operably linked to a plant-expressible promoter, and optionally to a 3′ end region involved in transcription termination and/or polyadenylation, wherein said enzyme comprising sedoheptulose 1,7-bisphosphatase activity comprises an amino acid sequence having at least 95% identity to the amino acid sequence of SEQ ID No. 2;
- and
- b. one or more nucleic acids encoding one or more polypeptides having the enzymatic activity of glycolate dehydrogenase operably linked to a plant-expressible promoter, and optionally to a 3′ end region involved in transcription termination and/or polyadenylation, wherein said one or more polypeptides are located in chloroplasts of said plants.
Dependencies are inferred from the claim text.