Modified microorganism with improved biomass separation behaviour
AssigneeBASF SE
InventorsJoanna Martyna Krawczyk, Stefan Haefner, Hartwig Schröder, Esther Dantas Costa, Oskar Zelder, Gregory Von Abendroth, Christoph Wittmann, René Stellmacher, Judith Becker
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Abstract
The present invention relates to a modified microorganism having, compared to its wild-type, a reduced activity of the enzyme that is encoded by the wcaJ-gene. The present invention also relates to a method for producing an organic compound and to the use of a modified microorganism.
Claims12
A modified microorganism having, compared to its wild-type, a deletion of the wcaJ-gene or an introduction of at least one mutation into the wcaJ-gene that leads to the expression of a truncated enzyme encoded by the wcaJ-gene in which at least 100 amino acids of the wild-type enzyme encoded by the wcaJ-gene are deleted from the C-terminal end;
- wherein the wild-type from which the modified microorganism has been derived belongs to the family of Pasteurellaceae and wherein the wild-type refers to the naturally occurring microorganism that has not been genetically modified,
- wherein the wcaJ-gene in the wildtype comprises a nucleic acid
- encoding an amino acid sequence which is at least 95% identical to SEQ ID NO:
- 4 over the total length of the amino acid sequence of SEQ ID NO:
- 4.
Dependencies are inferred from the claim text.