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US 10,273,465 B2Granted
Glycosyl hydrolase enzymes and uses thereof
AssigneeDanisco US Inc.
InventorsBenjamin Bower, Megan Yee Hsi, Thijs Kaper, Bradley R. Kelemen, Suzanne E. Lantz, Edmund A. Larenas, Colin Mitchinson, Steven Kim, William D. Hitz, Mark Emptage, Keith Dumont Wing
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Abstract
The present disclosure is generally directed to glycosyl hydrolase enzymes, compositions comprising such enzymes, and methods of using the enzymes and compositions, for example for the saccharification of cellulosic and hemicellulosic materials into sugars.
Claims12
A non-naturally occurring composition comprising:
- (a) a first polypeptide having f3-xylosidase activity, wherein the first polypeptide has at least 90% sequence identity to the amino acid sequence of SEQ ID NO:28 or the amino acid sequence corresponding to residues (i) 20-341, (ii) 21-350, (iii) 107-341, or (iv) 107-350 of SEQ ID NO:28;
- and
- (b) a second polypeptide having xylanase activity;
- wherein the non-naturally occurring composition comprises one or more polypeptides produced from a host cell, wherein the host cell is engineered to express the first polypeptide having β-xylosidase activity and wherein the first polypeptide is heterologous to the host cell.
1 more element
Dependencies are inferred from the claim text.