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US 10,273,449 B2Granted
Induction medium and methods for stem cell culture and therapy
AssigneeSanBio, Inc.
InventorAline Betancourt
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Abstract
Novel MSC stem-cell culture and therapy methods and culture medium compositions for the purpose of inducing, activating, or priming discrete uniform cell phenotypes to selectively promote or suppress inflammation and immunity, yielding polarized, primed, activated, or induced cells used in cell-based therapy.
Claims14
A cell culture comprising:
- (a) multipotent stem cells;
- and
- (b) culture medium comprising:
- (i) a Toll-like receptor-4 ligand (TLR4 ligand) at a concentration from about 100 pg/ml to about 10 μg/ml, and
- (ii) erythropoietin;
- wherein the cell culture is present in a hypoxic environment.
3 more elements
Dependencies are inferred from the claim text.