Microbial approach for the production of 5-hydroxytryptophan
AssigneeUniversity of Georgia Research Foundation, Inc.
InventorsYajun Yan, Yuheng Lin
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Abstract
5-hydroxytryptophan (5-HTP), a precursor of serotonin, is produced in a microbial host cell. A modified bacterial phenylalanine 4-hydroxylase (P4H) catalyzes the tryptophan 5-hydroxylation reaction. Optionally the host cell includes a cofactor regeneration mechanism, allowing continuous production of 5-HTP without supplementation of exogenous cofactors.
Claims24
A genetically engineered bacterial cell comprising a modified bacterial phenylalanine-4-hydroxylase (P4H) wherein the modified P4H catalyzes the 5-hydroxylation of tryptophan in the presence of a tetrahydromonapterin (MH4) cofactor, and in the absence of a tetrahydrobiopterin (BH4) cofactor or 6,7-dimethyl-5,6,7,8-tetrahydropterine hydrochloride, wherein the modified bacterial P4H comprises an amino acid mutation at any one, any two, or all three of amino acid positions 98, 179, and 231 of X. campestris P4H (SEQ ID NO:1), or at any one, any two, or all three corresponding amino acid positions in a bacterial P4H enzyme from another species.
Dependencies are inferred from the claim text.