Microorganism capable of simultaneous co-fermentation of mixed sugars and production method of butanol using the same
AssigneeGs Caltex Corporation
InventorSang-Hyun Lee
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Abstract
Embodiments of the invention provide a microorganism capable of simultaneous co-fermentation of two or more sugars in a lignocellulosic hydrolysate and having tolerance against microorganism growth inhibitory substances in the lignocellulosic hydrolysate and further having butanol productivity. In addition, embodiments of the invention provide a recombinant microorganism in which a pathway converting butyryl-CoA into butanol or a pathway converting butyrate into butyryl-CoA is promoted, and butanol productivity is increased. Further, a method for producing butanol using the microorganisms is provided.
Claims15
A microorganism capable of simultaneous co-fermentation of two or more sugars in a lignocellulosic hydrolysate and having the ability to produce butanol, wherein the microorganism is Clostridium acetobutylicum TM2-1-C(accession number KCTC 12604BP).
A recombinant microorganism having improved butanol productivity in comparison to Clostridium acetobutylicum TM2-1-C, wherein
- the recombinant microorganism is prepared from the Clostridium acetobutylicum TM2-1-C by promoting a pathway converting butyryl-CoA into butanol or a pathway converting butyrate into butyryl-CoA,
- the pathway converting butyryl-CoA into butanol is promoted by increasing aldehyde/alcohol dehydrogenase activity in comparison to Clostridium acetobutylicum TM2-1-C by introduction of an adhE gene encoding an aldehyde/alcohol dehydrogenase, and
- the pathway converting butyrate into butyryl-CoA is promoted by increasing CoA transferase activity in comparison to Clostridium acetobutylicum TM2-1-C by introduction of a ctfAB gene encoding a CoA transferase.
Dependencies are inferred from the claim text.