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US 10,266,834 B2Granted
Recombinant RNA particles and methods of producing proteins
AssigneeSynthetic Genomics, Inc.
InventorsRobert C. Brown, Kurt I. Kamrud
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AssigneeSynthetic Genomics, Inc.
InventorsRobert C. Brown, Kurt I. Kamrud
Estimating the term…
The present invention provides compositions and methods for the production and delivery of recombinant double-stranded RNA molecules (dsRNA) encoding heterologous proteins, which can be useful for various therapeutic purposes as well as for the production of desired proteins. The compositions contain engineered double-stranded RNA particles (dsRPs) that can contain a double-stranded RNA molecule that can be a genome or portion of a genome, which can be enclosed in a capsid or coat protein. The dsRNA molecule also comprises an RNA sub-sequence that encodes a heterologous protein. The dsRPs can be derived from wild-type viral organisms. The delivery of the dsRPs (or DNA or RNA molecules) of the invention to an organism provides for therapeutic benefits as well as for the production of desired proteins.
A double-stranded RNA particle (dsRP) comprising
An RNA molecule translatable by a host cell and encoding at least one heterologous RNA sub-sequence that encodes a heterologous protein that is translated by the host cell cellular components, and wherein the recombinant dsRNA molecule has at least 90% sequence identity with a wild type L-A viral genome, not counting the RNA sub-sequence that encodes the functional protein, and encodes a gag protein and an RNA-dependent RNA polymerase, and is sufficient for autonomous replication in a yeast host cell.
A method of producing a protein product in a host cell comprising:
Dependencies are inferred from the claim text.