Human progenitor T-cells into NK cells
AssigneeSunnybrook Health Sciences Centre
InventorsJuan Carlos Zuniga-Pflucker, Geneve Awong, Ross La Motte-Mohs
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Abstract
Human progenitor T cells that are able to successfully engraft a murine thymus and differentiate into mature human T and NK cells are described. The human progenitor T cells have the phenotype CD34+CD7+CD 1a−CD5− or CD34+CD7+CD1a−CD5+ and are derived from human hematopoietic stem cells, embryonic stem cells and induced pluripotent stem cells by coculture with cells expressing a Notch receptor ligand (OP9-DL1 or OP9-DL4). Such cells are useful in a variety of applications including immune reconstitution, the treatment of immunodeficiencies and as carriers for genes used in gene therapy.
Claims5
A method of generating natural killer (NK) cells comprising a) preparing T progenitor cells having the phenotype CD34+CD7+CD5+CD1a− by culturing stem cells or progenitor cells selected from the group consisting of hematopoietic stem cells, embryonic stem cells and induced pluripotent stem cells with stromal cells and isolating the progenitor T cells, b) culturing the isolated T progenitor cells with IL-15 or IL-2 and c) isolating NK cells.
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