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US 10,265,696 B2Granted
Nucleic acid amplification disk apparatus using temperature sensitive polymer synthesis and the analysis method using the same
AssigneesResearch & Business Foundation Sungkyunkwan University, CDgene Inc.
InventorJae Chern Yoo
Life of this patent
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Abstract
A nucleic acid amplification disk apparatus using a temperature sensitive polymer synthesis and the analysis method using the same, and more specifically, and the nucleic acid amplification device, and the analysis method using the nucleic acid amplification disk unit and the nucleic acid amplification disk for amplifying the Bacterial DNA or RNA, and the driving control section for controlling the nucleic acid amplification disk.
Claims34
A nucleic acid amplification disk apparatus, comprising:
- a nucleic acid amplification disk, comprising:
- a temperature sensitive synthetic polymer which is a polymer coated with or having absorbed a temperature sensitive fluorescence dye, wherein a fluorescent intensity emitted from the temperature sensitive fluorescence dye is changed according to a temperature,
- a first black film and a second black film both configured to be heated by a laser beam,
- a nucleic acid amplification chamber configured to amplify DNA by isothermal amplification or polymerase chain reaction, in which the temperature sensitive synthetic polymer is coated on one surface of the nucleic acid amplification chamber, and the first black film is coated on another surface of the nucleic acid amplification chamber,
- a preparation chamber configured to get prepare a DNA sample by cell lysis process,
- sub-chambers necessary for nucleic acid amplification,
- a fluid hole and a channel for connecting the sub-chambers,
- a double-sided adhesive tape comprising a first opening of the fluid hole, and
- a substrate comprising a second opening of the fluid hole, which is aligned with the first opening of the fluid hole,
- wherein the second black film is disposed between the double-sided adhesive tape and the substrate such that the second black film blocks a fluid path between the first opening and the second opening, and
- wherein the fluid path between the first opening and the second opening is configured to be connected when the second black film is melted by a heat of the laser beam;
- and
- a driving controller configured to drive the nucleic acid amplification disk, comprising:
- a motor configured to rotate the nucleic acid amplification disk,
- a laser beam generator configured to generate the laser beam, and
- sensors configured to measure temperature of the nucleic acid amplification chamber by sensing the florescent intensity emitted from the temperature sensitive florescent dye in the nucleic acid amplification chamber.
13 more elements
A nucleic acid amplification disk apparatus, comprising:
- a nucleic acid amplification disk, comprising:
- a temperature sensitive synthetic polymer which is a polymer coated with or having absorbed a thermochromic ink made by mixing temperature sensitive pigment, wherein a color of the thermochromic ink is changed according to a temperature,
- a first black film and a second black film both configured to be heated by a laser beam, a nucleic acid amplification chamber configured to amplify DNA by isothermal amplification or polymerase chain reaction, in which the temperature sensitive synthetic polymer is coated on one surface of the nucleic acid amplification chamber, and the first black film is coated on another surface of the nucleic acid amplification chamber,
- a preparation chamber configured to prepare a DNA sample by cell lysis process,
- sub-chambers necessary for nucleic acid amplification,
- a fluid hole and a channel for connecting the sub-chambers,
- a double-sided adhesive tape comprising a first opening of the fluid hole, and
- a substrate comprising a second opening of the fluid hole, which is aligned with the first opening of the fluid hole,
- wherein the second black film is disposed between the double-sided adhesive tape and the substrate such that the second black film blocks a fluid path between the first opening and the second opening, and
- wherein the fluid path between the first opening and the second opening is configured to be connected when the second black film is melted by a heat of the laser beam;
- and
- a driving controller configured to drive the nucleic acid amplification disk, comprising:
- a motor configured to rotate the nucleic acid amplification disk,
- a laser beam generator configured to generate the laser beam, and
- sensors configured to measure temperature of the nucleic acid amplification chamber by sensing the color of the thermochromic ink.
12 more elements
Dependencies are inferred from the claim text.