High efficiency, ultra-stable, bonded hydrophilic interaction chromatography (HILIC) matrix on superficially porous particles (SPPS)
AssigneeAzyp, LLC
InventorsDaniel W. Armstrong, Zachry S. Breitbach
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Abstract
The present invention relates to superficially porous particles (SPPs), also called core-shell, porous shell or fused core particles, which are state-of-the-art support materials used in the production of HPLC columns. Hydrolytically stable, highly selective superficially porous particle (SPP) hydrophilic interaction liquid chromatographic (HILIC) stationary phases having higher efficiencies and shorter retention times than analogous stationary phases on fully porous particles (FPP) is provided.
Claims16
Covalently bonded ultra-stable hydrophilic interaction chromatography (HILIC) phases comprising a superficially porous particle (SPP) support linked to a nonpolymeric HILIC selector via ether, carbamate, thioether, thiocarbamate, ester, triazole, or urea linkages,
- wherein the nonpolymeric HILIC selector is selected from the group consisting of carbohydrates and derivatives, oligosaccharides and derivatives, cyclic and derivatives oligosaccharides, peptides and derivatives, glycopeptides and derivatives, macrocyclic glycopeptides and derivatives, charged or ionic type, and zwitterionic type.
A superficially porous particle (SPP) comprising high efficiency, ultra-stable hydrophilic interaction chromatography (HILIC) matrix covalently bonded thereto via ether, carbamate, thioether, thiocarbamate, ester, triazole, or urea linkages, wherein the matrix comprises nonpolymeric HILIC selector selected from the group consisting of cyclodextrins, derivatized cyclodextrin, cyclofructans, derivatized cyclofructans, teicoplanin, vancomycin, teicoplanin aglycone, ristocetin A, dalbavancin, boromycin, sulfonated cyclofructans, sulfonated cyclodextrins, and 3-P,P-diphenylphosphoniumpropylsulfonate.
A hydrolytically stable highly selective superficially porous particle (SPP) hydrophilic interaction liquid chromatography (HILIC) phases having higher efficiencies and shorter retention times than analogous stationary phases on fully porous particles (FPP), wherein the HILIC packing material is not unmodified silica thereby protecting the SPP surface from dissolution and having broader HILIC selectivity and higher efficiencies than bare supports and fully porous supports comprising silica.
Dependencies are inferred from the claim text.