Recombinant Listeria vaccine strains and methods of producing the same
AssigneeAdvaxis, Inc.
InventorsAnu Wallecha, Robert Petit
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Abstract
The present invention provides methods of treating, protecting against and inducing an immune response against a tumor or cancer, comprising the step of administering to a subject a recombinant Listeria strain. In one embodiment the present invention relates to a recombinant Listeria strain, said recombinant Listeria strain comprising a recombinant nucleic add, said nucleic add comprising a first open reading frame encoding a recombinant polypeptide comprising a first N-terminal fragment of an LLO protein fused to a heterologous antigen or fragment thereof, and wherein said recombinant nucleic add further comprises a second open reading frame encoding a mutant PrfA protein.
Claims32
A recombinant Listeria strain, said recombinant Listeria strain comprising an episomal plasmid comprising a recombinant nucleic acid, said nucleic acid comprising a first open reading frame encoding a recombinant polypeptide comprising an N-terminal fragment of a listerioly sin O (LLO) protein fused to a heterologous antigen, and wherein said recombinant nucleic acid further comprises a second open reading frame encoding a mutant PrfA protein comprising an amino acid sequence of SEQ ID NO:
- 34.
A method of inducing an immune response against a tumor or a cancer in a human subject, the method comprising the step of administering to said subject a recombinant Listeria strain comprising an episomal plasmid comprising a recombinant nucleic acid, said nucleic acid comprising a first open reading frame encoding a recombinant polypeptide comprising an N-terminal fragment of a listerioly sin O (LLO) protein fused to a heterologous antigen, wherein said recombinant nucleic acid further comprises a second open reading frame encoding a mutant PrfA protein comprising an amino acid sequence of SEQ ID NO:34, thereby inducing an immune response against a tumor or a cancer.
Dependencies are inferred from the claim text.