Method for extracting and refining alkaloids from ipecac

A method for extracting and refining alkaloids from ipecac, the method comprising steps of:

• (1) extraction and concentration:
• grinding medicinal material of ipecac, and adding an acidic methanol solution or an acidic ethanol solution for extraction to give an extraction solution A;
• and concentrating the extraction solution A under a reduced pressure to give a concentrated solution B;
• (2) separation and enrichment:
• subjecting the concentrated solution B to a reversed-phase polymer filler J for adsorption;
• and performing desorption after the adsorption is completed, wherein water washing is performed, a washing solution C is collected, then eluting is performed with an alcoholic solution E, and a desorption solution D is collected;
• (3) purification:
• injecting the washing solution C into a preparative high performance liquid chromatograph for separation and purification, and collecting a solution G according to a corresponding spectrum band in a detection chromatogram of cephaeline hydrochloride;
• and injecting the desorption solution D into the preparative high performance liquid chromatograph for separation and purification, and collecting a solution H and a solution I according to corresponding spectrum bands in detection chromatograms of emetine hydrochloride and AIDS031406 hydrochloride, respectively;
• and
• (4) enrichment and drying:
• concentrating the solution G, solution H and solution I, which are then subjected to a reversed-phase polymer filler K for adsorption respectively;
• eluting, after the adsorption is completed, the solutions respectively with an alcoholic solution F;
• concentrating eluates, obtained after eluting, under a reduced pressure to dryness;
• and then performing vacuum drying to obtain pure cephaeline hydrochloride, pure emetine hydrochloride and pure AIDS031406 hydrochloride.