Method for producing beta-santalene
AssigneeFirmenich SA
InventorMichel Schalk
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Abstract
The present invention provides a method of producing β-santalene, said method comprising contacting at least one polypeptide with farnesyl pyrophosphate (FPP). In particular, said method may be carried out in vitro or in vivo to produce β-santalene, a very useful compound in the fields of perfumery and flavoring. The present invention also provides the amino acid sequence of a polypeptide useful in the method of the invention. A nucleic acid encoding the polypeptide of the invention and an expression vector containing said nucleic acid are also part of the present invention. A non-human host organism or a cell transformed to be used in the method of producing β-santalene is also an object of the present invention.
Claims19
An isolated nucleic acid molecule comprising
- a) the cDNA sequence of SEQ ID NO:
- 14 or the full-length complement thereof;
- b) a modified sequence of SEQ ID NO:
- 14, wherein the first 23 codons of SEQ ID NO:
- 14 were removed;
- or
- c) the modified sequence of b) optimized for E. coli expression.
Dependencies are inferred from the claim text.