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US 10,247,729 B2Granted
Media elaborated with newly synthesized antibodies (MENSA) and uses thereof
AssigneeMicrobplex, Inc.
InventorsFrances Eun-Hyung Lee, John L. Daiss
Life of this patent
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Abstract
Disclosed are methods and kits for early detection of antigen exposure through the presence or absence of antigen-specific antibodies.
Claims7
An analytical matrix comprising media elaborated with newly synthesized antibodies (MENSA) from recently proliferated antibody secreting cells (ASC) circulating in the blood;
- wherein the analytical matrix comprises at least a 106 or 107-fold reduction of contaminating pre-existing plasma antibodies relative to whole blood, plasma, or peripheral blood mononuclear cells (PBMC).
A method of producing an analytical matrix comprising MENSA the method comprising obtaining whole blood or PBMC from a subject separating plasma from the whole blood or PBMC to produce separated cells;
- isolating newly proliferated ASC from the separated cells;
- washing the newly proliferated ASC;
- and incubating the washed newly proliferated ASC in a media that supports maintenance of ASC and antibody secretion to thereby produce the MENSA;
- wherein the the analytical matrix comprises at least a 106 or 107-fold reduction of contaminating pre-existing plasma antibodies relative to whole blood, plasma, or PBMC.
1 more element
Dependencies are inferred from the claim text.