Backflush methods and devices for chromatography
AssigneePerkinElmer Health Sciences, Inc.
InventorAndrew Tipler
Life of this patent
Estimating the term…
Abstract
Certain embodiments described herein are directed to chromatography systems that include a microfluidic device and that implement one or more methods to direct sample to a desired fluid flow path. The methods can be used to backflush a sample to a desired fluid flow path to select certain analytes within a sample.
Claims18
A kit comprising:
- a microfluidic device comprising a laminated wafer body comprising an input port, a first outlet port and a second outlet port, in which each of the first outlet port and the second outlet port is fluidically coupled to the input port through an internal microchannel within the laminated wafer body, the microfluidic device configured to provide a sample from the input port to the first outlet port and to the second outlet port;
- a first fluid flow path fluidically coupled to the first outlet port of the microfluidic device to receive the sample from the first outlet port, wherein the first fluid flow path comprises a first restrictor configured to be fluidically coupled to a pressure source;
- a second fluid flow path fluidically coupled to the second outlet port of the microfluidic device to receive the sample from the second outlet port, wherein the second fluid flow path comprises a second restrictor configured to be fluidically coupled to a second pressure source;
- and
- instructions for using the microfluidic device, the pressure source and the second pressure source to backflush the sample in the second fluid flow path to the first fluid flow path through the laminated wafer body of the microfluidic device.
A kit comprising:
- a microfluidic device comprising a laminated wafer body comprising an input port, a first outlet port and a second outlet port, in which each of the first outlet port and the second outlet port is fluidically coupled to the input port through an internal microchannel within the laminated wafer body, the microfluidic device configured to provide a sample from the input port to the first outlet port and to the second outlet port
- a first fluid flow path fluidically coupled to the first outlet port of the microfluidic device to receive the sample from the first outlet port, wherein the first fluid flow path comprises a first restrictor configured to be fluidically coupled to a pressure source;
- a second fluid flow path fluidically coupled to the second outlet port of the microfluidic device to receive the sample from the second outlet port, wherein the second fluid flow path comprises a second restrictor configured to be fluidically coupled to a second pressure source;
- a first ferrule and a second ferrule each configured to provide fluidic coupling between the microfluidic device and one of the first fluid flow path and the second fluid flow path;
- and
- instructions for using the microfluidic device, the pressure source and the second pressure source to backflush the sample in the second fluid flow path to the first fluid flow path through the laminated wafer body of microfluidic device.
A system comprising a microfluidic device comprising a laminated wafer body comprising an input port, a first outlet port and a second outlet port, in which each of the first outlet port and the second outlet port is fluidically coupled to the input port through an internal microchannel within the laminated wafer body, the microfluidic device configured to provide a sample from the input port to the first outlet port and to the second outlet port;
- a first pressure source;
- a first fluid flow path fluidically coupled to the first outlet port of the microfluidic device to receive the sample from the first outlet port, wherein the first fluid flow path comprises a first restrictor configured to be fluidically coupled to the first pressure source;
- a second pressure source;
- and
- a second fluid flow path fluidically coupled to the second outlet port of the microfluidic device to receive the sample from the second outlet port, wherein the second fluid flow path comprises a second restrictor configured to be fluidically coupled to the second pressure source, wherein the first pressure source and the second pressure source are each configured to alter pressure to backflush sample in the second fluid flow path through the laminated wafer body of the microfluidic device and into the first fluid flow path.
Dependencies are inferred from the claim text.