# US 10,246,754 B1 · Molecular markers linked to disease resistance in soybean

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- Number: US 10,246,754 B1
- Document ID: US10246754B1
- Status: Granted
- Kind: B1
- Application number: 15/378,394
- Filed: 2016-12-14
- Granted: 2019-04-02
- Earliest claimed: 2014-04-25
- Assignees: Syngenta Participations AG
- Applicants: Syngenta Participations AG
- Inventors: Anderson Rotter Meda; Becky Welsh Breitinger
- Web page: https://patentgrep.com/patent/US10246754B1

## CPC

- C12Q 1/6895: for plants, fungi or algae
- A01H 1/02: Methods or apparatus for hybridisation; Artificial pollination {; Fertility}
- A01H 1/045: {using molecular markers}
- A01H 6/542: {Glycine max [soybean]}
- C12Q 2600/13: Plant traits
- C12Q 2600/156: Polymorphic or mutational markers
- C12Q 2600/158: Expression markers
- C12Q 2600/172: Haplotypes
- C12Q: MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS (immunoassay G01N33/53); COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- A01H: NEW PLANTS OR {NON-TRANSGENIC} PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES

## Abstract

The present invention relates to methods and compositions for identifying, selecting and/or producing a soybean plant or germplasm having a Rpp4 resistance allele and resistance to Phakosora pachyrhizi. A soybean plant, part thereof and/or germplasm that has been identified, selected and/or produced by any of the methods of the present invention is also provided. Also provided are single nucleotide polymorphisms (SNPs) associated with resistance to pathogens; and compositions including amplification primer pairs capable of initiating DNA polymerization by a DNA polymerase on soybean nucleic acid templates to generate soybean marker amplicons.

## Claims

### Claim 1

A method of producing a soybean plant having an Asian soybean rust (ASR) resistance, the method comprising the steps of:
a) isolating a nucleic acid from a soybean plant cell;
b) detecting in the nucleic acid of a) the presence of a haplotype associated with an ASR resistance allele, wherein said haplotype comprises a C at nucleotide 1 of SEQ ID NO: 1, a G at nucleotide 14 of SEQ ID NO: 1, a T at nucleotide 51 of SEQ ID NO: 1, a G at nucleotide 59 of SEQ ID NO: 1, a C at nucleotide 143 of SEQ ID NO: 1, a G at nucleotide 148 of SEQ ID NO: 1, and an A at nucleotide 157 of SEQ ID NO: 1;
c) identifying a first soybean plant on the basis of the presence of the haplotype detected in b);
d) crossing the first soybean plant with a second soybean plant not comprising said haplotype; and
e) producing a progeny plant from the cross of d), wherein said progeny plant comprises the haplotype detected in b), thereby producing a soybean plant having ASR resistance.