# US 10,246,734 B2 · System and method for detecting alpha-methylacyl-CoA racemase (AMACR) and prostate cancer > Agents: this data is available as JSON from the patentgrep API and MCP server — see https://patentgrep.com/llms.txt - Number: US 10,246,734 B2 - Document ID: US10246734B2 - Status: Granted - Kind: B2 - Application number: 15/369,611 - Filed: 2016-12-05 - Published: 2017-10-19 - Granted: 2019-04-02 - Earliest claimed: 2012-08-24 - Assignees: Case Western Reserve University - Applicants: Case Western Reserve University - Inventors: Chung-Chiun Liu; Laurie Dudik; Po-Yuam Lin - Web page: https://patentgrep.com/patent/US10246734B2 ## CPC - C12Q 1/533: involving isomerase - G01N 27/3272: {Test elements therefor, i.e. disposable laminated substrates with electrodes, reagent and channels (optical biosensors G01N33/52)} - H01M 14/00: Electrochemical current or voltage generators not provided for in groups H01M6/00 - H01M12/00; Manufacture thereof - G01N 2800/342: Prostate diseases, e.g. BPH, prostatitis - G01N 2800/7028: Cancer - C12Q: MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS (immunoassay G01N33/53); COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES - G01N: INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES (measuring or testing apparatus or processes other than immunoassay, involving enzymes or microorganisms C12M, C12Q) - H01M: PROCESSES OR MEANS, e.g. BATTERIES, FOR THE DIRECT CONVERSION OF CHEMICAL ENERGY INTO ELECTRICAL ENERGY ## Abstract A detection system for determining alpha-methylacyl-CoA (AMACR) levels in a bodily sample includes at least one reaction solution for generating H2O2 upon combination with AMACR in the bodily sample and a biosensor for determining a level of generated H2O2. The reaction solution includes a (2R)-2-methylacyl-CoA epimer that can be chirally inverted by AMACR to a (2S)-2-methylacyl-CoA epimer and an enzyme that carries out beta oxidation with the (2S)-2-methylacyl-CoA epimer to generate hydrogen peroxide (H2O2). ## Claims ### Claim 1 A detection system device for determining alpha-methylacyl-CoA (AMACR) levels in a bodily sample, comprising: at least one reaction solution for generating H2O2 upon combination with AMACR in the bodily sample, the reaction solution including a (2R)-2-methylacyl-CoA epimer that can be chirally inverted by AMACR to a (2S)-2-methylacyl-CoA epimer and peroxisomalacyl-coenzyme A oxidase 3 (ACOX3) that carries out beta oxidation with the (2S)-2-methylacyl-CoA epimer to generate hydrogen peroxide (H2O2); and a biosensor for determining the level of generated H2O2. ### Claim 2 (depends on 1) The detection system device of claim 1, the reaction solution including coenzyme A (CoA), peroxisomalacyl-coenzyme A oxidase 3 (ACOX3), adensonsine triphosphate (ATP), and a branched fatty acid with (R) and (S) epimers of which only the (R) epimer is a reaction substrate for AMACR. ### Claim 3 (depends on 2) The detection system device of claim 2, the branched fatty acid comprising pristanic acid. ### Claim 4 (depends on 1) The detection system device of claim 1, the bodily sample comprising a bodily fluid selected from the group consisting of blood, plasma, sera, and urine. ### Claim 5 (depends on 1) The detection system device of claim 1, the biosensor including a working electrode and a counter electrode, the working electrode and counter electrode including catalyst particles for the increasing the rate of electrochemical oxidation-reduction reaction with H2O2 and providing the detection of H2O2 at a lower oxidation potential than without the presence of the catalyst particles. ### Claim 6 (depends on 5) The detection system device of claim 5, the catalyst particles comprising nanoparticle metallic catalysts. ### Claim 7 (depends on 5) The detection system device of claim 5, the catalyst particles comprising a unary metal (M), a binary metal (M-X), a unary metal oxide (MOy), a binary metal oxide (MOyXOy), a metal-metal oxide composite material (M-MOy) or a combination of which, wherein y is less than 3, and Mand X are independently selected from a group consisting of Li, Na, Mg, Al, K, Ca, Cr, Mn, Fe, Co, Ni, Cu, Zn, Ga, Sr, Y, Zr, Nb, Mo, Ru, Rh, Pd, Ag, Cd, In, Sn, Ba, La, Ce, Pr, Nd, Sm, Eu, Gd, Tb, Dy, Ho, Er, Tm, Lu, Ta, W, Os, Ir, Pt, Au, and Pb. ### Claim 8 (depends on 5) The detection system device of claim 5, the catalyst particles comprising iridium oxide particles. ### Claim 9 (depends on 5) The detection system device of claim 5, further comprising a measuring device for applying voltage potentials to the working electrode and counter electrode and measuring the current flow between the working electrode and counter electrode. ### Claim 10 (depends on 1) The detection system device of claim 1, being suitable for detection of prostate cancer in a subject. ### Claim 11 An assay device for detecting prostate cancer in a subject comprising: at least one reaction solution for generating H2O2 upon combination with AMACR in a bodily sample obtained from a subject, the reaction solution including a (2R)-2-methylacyl-CoA epimer that can be chirally inverted by AMACR to a (2S)-2-methylacylCoA epimer and peroxisomalacyl-coenzyme A oxidase 3 (ACOX3) that carries out beta oxidation with the (2S)-2-methylacyl-CoA epimer to generate hydrogen peroxide (H2O2); and a biosensor for determining the level of generated H2O2. ### Claim 12 (depends on 11) The assay device of claim 11, the reaction solution including coenzyme A (CoA), peroxisomalacyl-coenzyme A oxidase 3(ACOX3), adensonsine triphosphate (ATP), and a branched fatty acid with (R) and (S) epimers of which only the (R) epimer is a reaction substrate for AMACR. ### Claim 13 (depends on 12) The assay device of claim 12, the branched fatty acid comprising pristanic acid. ### Claim 14 (depends on 11) The assay device of claim 11, the bodily sample comprising a bodily fluid selected from the group consisting of blood, plasma, sera, and urine. ### Claim 15 (depends on 11) The assay device of claim 11, the biosensor including a working electrode and a counter electrode, the working electrode and counter electrode including catalyst particles for the increasing the rate of electrochemical oxidation-reduction reaction with H2O2 and providing the detection of H2O2 at a lower oxidation potential than without the presence of the catalyst particles. ### Claim 16 (depends on 15) The assay device of claim 15, the catalyst particles comprising nano-particle metallic catalysts. ### Claim 17 (depends on 15) The assay device of claim 15, the catalyst particles comprising a unary metal (M), a binary metal (M-X), a unary metal oxide (MOy), a binary metal oxide (MOy-XOy), a metalmetal oxide composite material (M-MOy) or a combination of which, wherein y is less than 3, and Mand X are independently selected from a group consisting of Li, Na, Mg, Al, K, Ca, Cr, Mn, Fe, Co, Ni, Cu, Zn, Ga, Sr, Y, Zr, Nb, Mo, Ru, Rh, Pd, Ag, Cd, In, Sn, Ba, La, Ce, Pr, Nd, Sm, Eu, Gd, Tb, Dy, Ho, Er, Tm, Lu, Ta, W, Os, Ir, Pt, Au, and Pb. ### Claim 18 (depends on 15) The assay device of claim 15, the catalyst particles comprising iridium oxide particles. ### Claim 19 (depends on 15) The assay device of claim 15, further comprising a measuring device for applying voltage potentials to the working electrode and counter electrode and measuring the current flow between the working electrode and counter electrode.