# US 10,246,722 B2 · Crop resistance to nematodes

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- Number: US 10,246,722 B2
- Document ID: US10246722B2
- Status: Granted
- Kind: B2
- Application number: 14/232,041
- Filed: 2012-07-13
- Published: 2014-10-02
- Granted: 2019-04-02
- Earliest claimed: 2011-07-13
- Assignees: The Curators of the University of Missouri; Cornell University; The United States of America as represented by the Secretary of Agriculture
- Applicants: Melissa G. Mitchum; Amy Replogle; Jianying Wang; Xiaohong Wang; Shiyan Chen; Ping Lang
- Inventors: Melissa G. Mitchum; Amy Replogle; Jianying Wang; Xiaohong Wang; Shiyan Chen; Ping Lang
- Web page: https://patentgrep.com/patent/US10246722B2

## CPC

- C12N 15/8285: {for nematode resistance}
- C12N 15/8218: {Antisense, co-suppression, viral induced gene silencing [VIGS], post-transcriptional induced gene silencing [PTGS]}
- C12N 15/8239: {pathogen inducible}
- Y02A 40/146: Genetically Modified [GMO] plants, e.g. transgenic plants
- C12N: MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA (microbiological testing media C12Q1/00)
- Y02A: TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE

## Abstract

Methods of inhibiting plant parasitic nematodes, methods of obtaining transgenic plants useful for inhibiting such nematodes, and transgenic plants that are resistant to plant parasitic nematodes through inhibition of plant nematode CLAVATA3/ESR (CLE) peptide receptor genes are provided. Methods for expressing genes at plant parasitic nematode feeding sites with plant nematode CLE peptide receptor gene promoters are also provided, along with nematode CLE peptide receptor gene promoters that are useful for expressing genes in nematode feeding sites as well as transgenic plants and nematode resistant transgenic plants comprising the promoters.

## Claims

### Claim 1

A method for inhibiting plant parasitic nematode damage to a soybean plant, said method comprising:
(1) reducing the expression of an endogenous plant RECEPTOR-LIKE PROTEIN KINASE 2 (RPK2)-like gene by expressing in said soybean plant RNAi to silence said RPK2-like gene, wherein said RPK2-like gene is set forth in SEQ ID NO:17, SEQ ID NO: 18, SEQ ID NO: 20 or SEQ ID NO: 21; and
(2) growing said soybean plant expressing said RNAi,
thereby inhibiting plant parasitic nematode damage to said soybean plant.

### Claim 2 (depends on 1)

The method of claim 1, further comprising reducing the expression of an endogenous CLAVATA2 (CLV2) gene by introducing into said soybean plant one or more non-natural mutations in said CLV2 gene, or by expressing in said soybean plant RNAi to silence said CLV2 gene.

### Claim 3 (depends on 1)

The method of claim 1, further comprising the step of harvesting a product of said soybean plant.

### Claim 4 (depends on 3)

The method of claim 3, wherein said product is a leaf, stem, flower, seed, root, or tuber.

### Claim 5 (depends on 3)

The method of claim 3, wherein the yield and/or quality of said product is increased relative to a control plant not expressing said RNAi.

### Claim 6 (depends on 1)

The method of claim 1, wherein said plant parasitic nematode is a cyst nematode.

### Claim 7 (depends on 6)

The method of claim 6, wherein said cyst nematode is a Heterodera spp.

### Claim 8 (depends on 6)

The method of claim 6, wherein said plant parasitic nematode is Heterodera glycines.

### Claim 9 (depends on 1)

A genetically modified nematode resistant soybean plant produced by the method of claim 1, wherein said soybean plant expresses said RNAi.