# US 10,246,717 B2 · Wheat with increased resistant starch levels

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- Number: US 10,246,717 B2
- Document ID: US10246717B2
- Status: Granted
- Kind: B2
- Application number: 15/975,410
- Filed: 2018-05-09
- Published: 2018-09-20
- Granted: 2019-04-02
- Earliest claimed: 2011-10-04
- Assignees: Arcadia Biosciences, Inc.
- Applicants: Arcadia Biosciences, Inc.
- Inventors: Ann J. Slade; Dayna L. Loeffler; Aaron M. Holm; Jessica C. Mullenberg
- Web page: https://patentgrep.com/patent/US10246717B2

## CPC

- C12N 15/8245: {involving modified carbohydrate or sugar alcohol metabolism, e.g. starch biosynthesis}
- A01H 6/4678: {Triticum sp. [wheat]}
- C12N 9/107: {1,4-Alpha-glucan branching enzyme (2.4.1.18)}
- C12Y 204/01018: 1,4-Alpha-glucan branching enzyme (2.4.1.18), i.e. glucan branching enzyme
- C12N: MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA (microbiological testing media C12Q1/00)
- A01H: NEW PLANTS OR {NON-TRANSGENIC} PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- C12Y: ENZYMES

## Abstract

A series of independent human-induced non-transgenic mutations found at one or more of the SBEII genes of wheat; wheat plants having these mutations in one or more of their SBEII genes; and a method of creating and finding similar and/or additional mutations of SBEII by screening pooled and/or individual wheat plants. The seeds and flour from the wheat plants of the present invention exhibit an increase in amylose and resistant starch without having the inclusion of foreign nucleic acids in their genomes. Additionally, the wheat plants of the present invention exhibit altered SBEII activity without having the inclusion of foreign nucleic acids in their genomes.

## Claims

### Claim 1

A wheat plant of the species Triticum turgidum L. ssp durum comprising one or more homozygous severe mutations in both SBEIIa alleles of each SBEIIa gene of the A and B genomes, wherein said wheat plant produces grain that germinates, and wherein the grain from said wheat plant has an increased amylose level as compared to grain from a wild type wheat plant.

### Claim 2 (depends on 1)

Wheat grain from the wheat plant of claim 1.

### Claim 3 (depends on 2)

Flour comprising a cell of the wheat grain of claim 2.

### Claim 4 (depends on 1)

A food or beverage product comprising a cell of the wheat plant of claim 1.

### Claim 5 (depends on 1)

A wheat seed, plant part, or progeny thereof from the wheat plant of claim 1.